Laboratory of Animal Model Organisms

 

 

Head:


Agata Tyczewska, PhD

 


   

Staff:

Joanna Gracz Bernaciak, PhD Eng.;

Karol Kołodziejczak, MSc Eng.

 

 

 

Laboratory affiliated to the Department of Integrative Biology

 

Keywords:

Caenorhabditis elegans, microinjection, Caenorhabditis elegans, C. elegans, MediaJet, Mediaclave, Tissue Lyser, PC-100 micro needle puller, nutrient media Integra

 

Offer:

  • Nutrient media sterilization (from 1 l to 10 l)
  • Petri plates pouring, up to 900 plates per hour (Ø 35 mm, 60 mm, 90 mm)
  • Microneedle preparation
  • Microinjections (possibility to use with C. elegans, cell nuclei and cytomplasm of adherent cells, pronucleuses of fertilized mouse oocytes, Xenopus laevis oocytes, fish embryos in early development phases)

 

Equipment:

  • Mediaclave 10, Integra
  • MediaJet vario, with sets for Ø 35 mm, 60 mm, 90 mm Petri plates, Integra
  • PC-100 microneedle puller, Narishige
  • Axio Vert.A1 microscope (Zeiss), with the Eppendorf microinjection set mounted:
    the InjectMan ®4 micromanipulator with dynamic motion control and the programmable FemtoJet ®4i micro diaphragm with integrated pressure supply
  • Tissue Lyser with 2 x 24 Adapter Set for 2 ml tubes, and Grinding Jar Set (stainless steel, 2 x 10 ml)