Department of Molecular Genetics


 

Head:
Prof. Piotr Kozłowski

 

 


Staff:

Prof. Marzena Wojciechowska      

Prof. Anna Jasińska - obecnie w Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, USA

Dr. Paulina Gałka-Marciniak

Dr. Martyna Urbanek- Trzeciak

Dr. Malwina Suszyńska

Dr. Karol Czubak

Katarzyna Chojnacka, MSc


PhD Students:

Paulina Nawrocka, MSc

 


 
 

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Key words:

cancer genetics, copy number variation polymorphism (CNV), multiplex ligation-dependent probe amplification (MLPA), miRNA, ncRNA, circRNA, ddPCR

 

Research area:

  • cancer genetics
  • development of technologies for CNV polymorphism genotyping,
  • genetic polymorphism and somatic variation of microRNA genes,
  • computational analysis of copy number variation polymorphism in the human genome and other eukaryotic genomes
  • circRNA expression level analysis in myotonic dystrophy (DM1)

 

Ongoing research projects:

1. Analysis of somatic mutations in miRNA and miRNA-biogenesis genes to identify new
therapeutic targets and biomarkers of cancer.

NSC – MAESTRO 8- 2016/22/A/NZ2/00184, PI: P. Kozłowski, Prof.

2. Analysis of the role of BARD1 gene in genetic predisposition to breast cancer - utilization of unique strategy of association analysis of founder mutations.

NSC – OPUS 9- 2015/17/B/NZ2/01182, PI: P. Kozłowski, Prof.

3. Understanding the mechanism of action for small molecule kinase inhibitors in alleviating myotonic dystrophy type 1 (DM1) pathomechanism.

NSC – OPUS 7- 2014/13/B/NZ5/03214, PI: M. Wojciechowska, Prof.

4. The APOBEC3B deletion - analysis of its role in familial breast cancer predisposition and determination of its consequences on mRNA level.

NSC – PRELUDIUM 10- 2015/19/N/NZ5/02247- PI: K. Klonowska, PhD 

5. Expression of circular RNA in myotonic dystrophy type 1 - implications to pathogenesis.

NSC – PRELUDIUM 11- 2016/21/N/NZ5/00508, PI - K. Czubak, PhD

6. Novel transcriptomic and proteomic biomarkers of DM1 pathogenesis via kinome analysis. European Union’s Seventh Framework Programme - Marie Curie Actions- PI: M. Wojciechowska, Prof.

7. Identification of somatic mutations within microRNA genes in cancer. Grant for Young Scientists, 2018, PI: P. Gałka- Marciniak, PhD

 

Selected publications

Suszynska M, Klonowska K, Jasinska AJ, Kozlowski P.Large-scale meta-analysis of mutations identified in panels of breast/ovarian cancer-related genes - Providing evidence of cancer predisposition genes. Gynecol Oncol. 2019 Feb 4. doi: 10.1016/j.ygyno.2019.01.027.

Czubak K, Taylor K, Piasecka A, Sobczak K, Kozłowska K, Philips A, Sedehizadeh S, Brook JD, Wojciechowska M, Kozlowski P. Global increase in circRNA levels in myotonic dystrophy. https://doi.org/10.1101/489070

Dabrowska M, Czubak K, Juzwa W, Krzyzosiak WJ, Olejniczak M, Kozlowski P. qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites. Nucleic Acids Res. 2018 Jun 7. doi: 10.1093/nar/gky505.

Klonowska K, Kluzniak W, Rusak B, Jakubowska A, Ratajska M, Krawczynska N, Vasilevska D, Czubak K, Wojciechowska M, Cybulski C, Lubinski J, Kozlowski P. The 30 kb deletion in the APOBEC3 cluster decreases APOBEC3A and APOBEC3B expression and creates a transcriptionally active hybrid gene but does not associate with breast cancer in the European population. Oncotarget. 2017 Jul 19;8(44):76357-76374.

Philips A, Stolarek I, Kuczkowska B, Juras A, Handschuh L, Piontek J, Kozlowski P, Figlerowicz M. Comprehensive analysis of microorganisms accompanying human archaeological remains. Gigascience. 2017 Jul 1;6(7):1-13.

Klonowska K, Czubak K, Wojciechowska M, Handschuh L, Zmienko A, Figlerowicz M, Dams-Kozlowska H, Kozlowski P. Oncogenomic portals for the visualization and analysis of genome-wide cancer data. Oncotarget. 2016 Jan 5;7(1):176-92.

Marcinkowska-Swojak M, Szczerbal I, Pausch H, Nowacka-Woszuk J, Flisikowski K, Dzimira S, Nizanski W, Payan-Carreira R, Fries R, Kozlowski P, Switonski M. Copy number variation in the region harboring SOX9 gene in dogs with testicular/ovotesticular disorder of sex development (78,XX; SRY-negative). Sci Rep. 2015 Oct 1;5:14696.

Czubak K, Lewandowska MA, Klonowska K, Roszkowski K, Kowalewski J, Figlerowicz M, Kozlowski P. High copy number variation of cancer-related microRNA genes and frequent amplification of DICER1 and DROSHA in lung cancer. Oncotarget. 2015 Sep 15;6(27):23399-416.

Klonowska K, Ratajska M, Czubak K, Kuzniacka A, Brozek I, Koczkowska M, Sniadecki M, Debniak J, Wydra D, Balut M, Stukan M, Zmienko A, Nowakowska B, Irminger-Finger I, Limon J, Kozlowski P. Analysis of large mutations in BARD1 in patients with breast and/or ovarian cancer: the Polish population as an example. Sci Rep. 2015 May 21;5:10424.

Marcinkowska-Swojak M, Klonowska K, Figlerowicz M, Kozlowski P. An MLPA-based approach for high-resolution genotyping of disease-related multi-allelic CNVs. Gene. 2014 Aug 10;546(2):257-62.