Department of Medical Biology


 

Head:
Prof. Mirosława Z. Naskręt-Barciszewska

 

 


Staff:
Dr. Agnieszka Belter

 Iwona Gawrońska

 

 


PhD Students:
Paweł Głodowicz, MSc. Eng.

 

 

 


 

Fig.1


 

Fig.2


 

Fig.3


 

 


Key words:

methylation of  DNA human, brain tumors, cancer, RNA ,modified nucleosides,  miRNA,  ribozymes

 

Scientific profile:

The activity of the Department team focuses on searching of new molecular markers of human diseases based on nucleic acids and their derivatives. We have analyzed the presence of some modified nucleosides [5-methyl cytosine (m5C) and 8-oxoguanosine (8-oxodG)] in DNA (Fig. 1) of various diseases as well as in DNA from different trees. Using microRNA arrays, we analyze the expression of miRNA in brain tumors with different degrees of malignancy. New miRNAs as marker Glioblastoma multiforme have been found (Fig. 3).

 

Current research activity

  • Synthesis and properties  of new ribozymes and DNAzymes.
  • Structure of miRNAs.
  • Analysis of 5-methylcytosine contents in human DNA of human with different pathologies.
  • Evaluation cytosine demethylation process in plant DNAs.
  • Analysis of the ncRNAs (BC200) in brain tumors.

 

Most important research achievements:

  • Identification of a correlation between the level of DNA methylation and malignancy of brain tumors (Fig. 2).
  • Finding of decreasing m5C level  in DNA of cryogenic seeds of trees.
  •  Polish Prime Minister Prize and Poznan City Mayor Science Prize for Dr A. Belter thesis - Structural aspects of miRNA specific for brain tumors. Supervised by MZ Naskręt-Barciszewska
  • Magister diploma for Karolina Majcherek – The structure and function of the brain cytoplasmic RNA BC200.

 

Selected publications:

  1. M. Michalak, M.Z. Barciszewska, J. Barciszewski, B.P. Plitta, P. Chmielarz (2013)  Global Changes in DNA Methylation in Seeds and Seedlings of Pyrus communis after Seed Desiccation and Storage.  PLoS One 8(8), e70693.
  2. MZ. Barciszewska, PP Perrigue, J Barciszewski. (2016)  tRNA – the golden standard in molecular biology. Molecular BioSystems. 12, 12-17.
  3. M. Olszewska, MZ. Barciszewska, M. Fraczek, N. Huleyuk, V B. Chernykh, D. Zastavna, J. Barciszewski, M. Kurpisz. (2016) Global methylation status of sperm DNA in carriers of chromosome structural aberrations Asian Journal of Andrology 18, 1-8.
  4. K. Rolle, M. Piwecka, A. Belter, D. Wawrzyniak, J. Jeleniewicz, MZ. Barciszewska, J. Barciszewski. (2016) The sequence and structure determine the function of mature human miRNAs". PLoS ONE 11(3): e0151246.
  5. B.P. Plitta, M. Michalak, M.Z. Naskręt-Barciszewska, J. Barciszewski, P Chmielarz (2014) DNA methylation of Quercus robur L. plumules following cryopretreatment and cryopreservation. Plant Cell Tiss Organ Cult 117:31-37.
  6. A-M. Barciszewska, S. Nowak, M.Z. Naskręt-Barciszewska. (2014)The Degree of Global DNA Hypomethylation in Peripheral Blood Correlates with that in Matched Tumor Tissues in Several neoplasia. PLoS One 9(3), e92599.
  7. A. Belter, D. Gudanis, K. Rolle, M. Piwecka,  Z. Gdaniec, MZ. Naskręt-Barciszewska, J. Barciszewski. (2014) Mature miRNAs form secondary structure, which suggests their function beyond RISC. PLoS One  9(11): e113848.
  8. A. Belter, MZ. Naskręt-Barciszewska. (2014) Developmentbof hammerhead ribozymes targeting miR-21 BioTechnologia 95;267-271.
  9. A. Belter, MZ. Naskręt-Barciszewska (2014) The correlation of structural features of mature miRNAs with their biological function. BioTechnologia, 95, 181-186 C.
  10. M. Doniak , MZ. Barciszewska, J. Kaźmierczak, A. Kaźmierczak. (2015) The crucial elements of the 'last step' of programmed cell death induced by kinetin in root cortex of V. faba ssp. minor seedlings. Plant Cell Rep DOI 10.1007/s00299-014-1681-9.
  11. A-M. Barciszewska,D. Gurda, P. Głodowicz, S. Nowak, MZ. Naskręt-Barciszewska (2015) A new epigenetic mechanism of temozolomide action in glioma cells. PloS ONE 10(8):e136669.
  12. A. Belter, K. Rolle, M. Piwecka, M. A. Fedoruk-Wyszomirska, MZ  Naskręt-Barciszewska, J.Barciszewski. (2016) Inhibition of miR-21 in glioma cells using catalytic nucleic acids" Scientific Reports. 6:24516.

 

Patents:

J. Barciszewski, M. Barciszewska, L. Rychlewski, E. Wyszko, I. Gawrońska, R. Żukiel, S. Nowak
Sequence of dsRNA: ATN-RNA specific for tenascin-C, United States Patent, 03.02.2015
Patent No.: US 8,946,400 B2

 

Patent applications:

Mirosława Zofia Naskręt-Barciszewska, Agnieszka Belter, Katarzyna Monika Rolle, Monika Piwecka, Patrycja Sosińska, Agnieszka Fedoruk-Wyszomirska.
Rybozymy typu hammerhead, kompozycja, środek terapeutyczny je obejmujące, ich zastosowania oraz sposób hydrolizy miR21 i prekursorów miR21,
Patent application (national) (P.403341), 2013

Mirosława Zofia Naskręt-Barciszewska, Agnieszka Belter, Katarzyna Monika Rolle, Monika Piwecka, Patrycja Sosińska, Agnieszka Fedoruk-Wyszomirska
Hammerhead ribozymes International application PCT (PCT/IB2014/060188), 2014