Department of Genome Engineering


 

Head:

Marta Olejniczak,  PhD

Associate Prof. IBCH PAS



 

Staff:

Dr. Paweł Śledziński,

Teresa Dymarek-Babś, MSc

Marianna Pewińska, MSc

Anna Misiukiewicz, MSc


 

 PhD Students:

Magdalena Dąbrowska,MSc

Anna Kotowska-Zimmer, MSc eng.

Mateusz Nowaczyk, MSc

 

 
 

 

Keywords:

genome editing, CRISPR/Cas9, Cpf1, genetic therapy, RNA interference, miRNA, Huntington’s disease

 

Research area:

Research in the Department of Genome Engineering is currently focused on three major areas:

- Development of genome editing technologies and methods for assessing its effectiveness and specificity

- The use of genome editing technology to establish disease models and in cell and pre-clinical therapy of genetic diseases

- The use of genetic tools in experimental therapy for polyglutamine diseases

 

Ongoing research projects:

1. The use of genetic tools in the experimental therapy of polyglutamine diseases.

NSC – SONATA BIS 5

 

Selected publications from the last three years:

  • Sledzinski P, Nowaczyk M and Olejniczak M*, Computational Tools and Resources Supporting CRISPR-Cas Experiments. Cells. 2020, 9, 1288
  • Dabrowska M, Ciolak A, Kozlowska E, Fiszer A and Olejniczak M*, Generation of New Isogenic Models of Huntington’s Disease Using CRISPR-Cas9 Technology. Int. J. Mol. Sci. 2020, 21(5), 1854
  • Kotowska-Zimmer A, Ostrovska Y and Olejniczak M*. Universal RNAi triggers for the specific inhibition of mutant huntingtin, atrophin-1, ataxin-3 and ataxin-7 expression. Mol Ther Nucl Acids 2020, 19:562-571.
  • Dabrowska M, and Olejniczak M*, Gene therapy for Huntington's disease using targeted endonucleases. Methods Mol Biol. 2020, 2056:269-284
  • Dabrowska M, Czubak K, Juzwa W, Krzyzosiak WJ, Olejniczak M*, Kozlowski P*. qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites. Nucleic Acids Res. 2018, 46(17):e101
  • Dabrowska M, Juzwa W, Krzyzosiak WJ, Olejniczak M* Precise excision of the CAG tract from the Huntingtin Gene by Cas9 Nickases, Front Neurosci. 2018, 12:75
  • Olejniczak M*, Kotowska-Zimmer A, Krzyzosiak WJ. Stress-induced changes in miRNA biogenesis and functioning, Cell Mol Life Sci., 2018, 75:177-191