Department of Genome Engineering

Prof. Dr. hab. Marta Olejniczak

Head of Department

marta.olejniczak@ibch.poznan.pl
ext. 1136

Research activity

Research area

Research in the Department of Genome Engineering is currently focused on three major areas:

Development of genome editing technologies and methods for assessing its effectiveness and specificity
The use of genome editing technology to establish disease models and in cell and pre-clinical therapy of genetic diseases
The use of genetic tools in experimental therapy for polyglutamine diseases

Keywords

genome editing,
CRISPR/Cas9,
Cpf1,
genetic therapy,
RNA interference,
miRNA,
Huntington’s disease

Research Projects

Assessing efficacy and safety of genome EDITing approaches for Sickle Cell Disease. EU - HORIZON EUROPE (MO)
Searching for new therapeutic targets in polyglutamine diseases. NCN – OPUS (MO)
Allele-selective therapy for polyglutamine diseases with the use of RNA interference technology. NCN- PRELUDIUM BIS (MO)
Microscopic identification of proteins involved in DNA repair of CAG repeats regions. NCN – PRELUDIUM (MN)
Investigation of DNA repair mechanisms in DRPLA model using CRISPR-Cas system. NCN - PRELUDIUM (MD)
The use of artificial miRNAs in experimental therapy of Huntington's Disease. NCN - PRELUDIUM (AKZ)
The importance of CAG repeat length polymorphism in the pathogenesis of cancer. MEiN - PERŁY NAUKI (MK)

Selected publications

Sledzinski P, Nowaczyk M, Smielowska MI and Olejniczak M* CRISPR/Cas9-induced double-strand breaks in the huntingtin locus lead to CAG repeat contraction through DNA end resection and homology-mediated repair. BMC Biology, 2024; 22:282
Kotowska-Zimmer A, Przybyl L, Pewinska M, Suszynska-Zajczyk J, Wronka D, Figiel M, and Olejniczak M* A CAG repeat-targeting artificial miRNA lowers the mutant huntingtin level in the YAC128 model of Huntington’s disease. Molecular Therapy Nucl. Acids, 2022; 28: 702-715
Karwacka M and Olejniczak M* Advances in modeling polyglutamine diseases using genome editing tools. Cells, 2022; 11, 517
Sledzinski P, Dabrowska M, Nowaczyk M, Olejniczak M*. Paving the way towards precise and safe CRISPR genome editing. Biotechnol Adv. 2021; 49:107737
Kotowska-Zimmer A, Pewinska M and Olejniczak M*, Artificial miRNAs as therapeutic tools: Challenges and opportunities. Wiley Interdiscip Rev RNA 2021; e1640
Sledzinski P, Nowaczyk M and Olejniczak M*, Computational Tools and Resources Supporting CRISPR-Cas Experiments. Cells. 2020, 9, 1288
Dabrowska M, Ciolak A, Kozlowska E, Fiszer A and Olejniczak M*, Generation of New Isogenic Models of Huntington’s Disease Using CRISPR-Cas9 Technology. Int J Mol Sci. 2020, 21, 1854
Kotowska-Zimmer A, Ostrovska Y and Olejniczak M*. Universal RNAi triggers for the specific inhibition of mutant huntingtin, atrophin-1, ataxin-3 and ataxin-7 expression. Mol Ther Nucl Acids 2020, 19:562-571.
Dabrowska M, and Olejniczak M*, Gene therapy for Huntington's disease using targeted endonucleases. Methods Mol Biol. 2020, 2056:269-284
Dabrowska M, Czubak K, Juzwa W, Krzyzosiak WJ, Olejniczak M*, Kozlowski P*. qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites. Nucleic Acids Res. 2018, 46:e101
Dabrowska M, Juzwa W, Krzyzosiak WJ, Olejniczak M* Precise excision of the CAG tract from the Huntingtin Gene by Cas9 Nickases, Front Neurosci. 2018, 12:75
Olejniczak M*, Kotowska-Zimmer A, Krzyzosiak WJ. Stress-induced changes in miRNA biogenesis and functioning, Cell Mol Life Sci., 2018, 75:177-191
Olejniczak M*, Urbanek M.O., Jaworska E, Witucki Ł, Szcześniak M.W., Makałowska I, Krzyzosiak WJ. Sequence-non-specific effects generated by various types of RNA interference triggers. BBA Gene Regul Mech. 2016, 1859:306-14
Olejniczak M*, Urbanek MU, Krzyzosiak WJ* The Role of the Immune System in Triplet Repeat Expansion Diseases. Mediators Inflamm. 2015, 873860,
Olejniczak M, Galka-Marciniak P, Polak K, Fligier A, Krzyzosiak WJ. RNAimmuno: A database of the nonspecific immunological effects of RNA interference and microRNA reagents. RNA 2012, 18:930-5
Olejniczak M, Polak K, Galka-Marciniak P, Krzyzosiak WJ. Recent advances in understanding of the immunological off-target effects of siRNA. Curr Gene Ther. 2011, 17

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Department of Genome Engineering

Researchers:

Technical Staff:

PhD students:

Research activity

Research area

Keywords

Research Projects

Selected publications

Adres

Address

Department of Genome Engineering

Researchers:

Technical Staff:

PhD students:

Research activity

Research area

Keywords

Rys. 1

Rys. 2

Rys. 3

Rys. 4

Research Projects

Selected publications

Adres

Address