Department of Biomedical Proteomics


 

Kierownik:

Magdalena Łuczak,PhD,

Associate Prof. ICHB PAS

 


 

Staff:

 

 


 

PhD Students:

Joanna Tracz, MSc Eng.


 


 
 

 

Rys.1

 

 

Rys.2

 

 

Rys.3

 

 

Rys.4

Keywords:

Proteomics, proteins, mass spectrometry, chronic kidney disease, cardiovascular disease, multiple myeloma, oxidative stress, inflammation

 

Research area:

- proteomic analysis of the molecular basis of atherosclerosis in chronic kidney disease

- analysis of the relationship between oxidative stress and chronic inflammation in chronic kidney disease and cardiovascular disease

- identification of protein biomarkers of resistance to chemotherapy

- analysis of the mechanism of cell resistance to proteasome inhibitors

- wide spectrum of technical approaches: electrophoresis, immunoenzymatic methods, liquid and gas chromatography, mass spectrometry, microscopy, bioinformatic analysis of proteomics data.

 

Ongoing research projects:

1. Molecular mechanism of atherosclerosis progression in chronic kidney disease.

NSC – OPUS 10

 

2. Proteomic analysis of myeloma plasma cells to determine proteins and pathways involved in acquired resistance to proteasome inhibitors.

NSC OPUS 



Selected publications from the last three years:

1. B. Swarcewicz, A. Sawikowska, Ł. Marczak, M. Łuczak, D. Ciesiołka, K. Krystkowiak,
A. Kuczynska, M. Pislewska-Bednarek, P. Krajewski, M. Stobiecki
Effect of drought stress on metabolite contents in barley recombinant inbred line population revealed by untargeted GC–MS profiling
Acta Physiol Plant
, 39; 158 (2017)

 

2. A. Zdarta, J. Tracz, M. Luczak, U. Guzik, E. Kaczorek
Hydrocarbon-induced changes in proteins and fatty acids profiles of Raoultella ornithinolytica M03.
Journal of Proteomics, 164; 43-51 (2017)

 

3. M. Luczak, T. Kubicki, Z. Rzetelska, T. Szczepaniak, Przybylowicz-Chalecka,
B. Ratajczak, J. Czerwinska-Rybak, A. Nowicki, M. Joks, A. Jakubowiak, M. Komarnicki,
D. Dytfeld
Comparative proteomic profiling of sera from patients with refractory multiple myeloma reveals potential biomarkers predicting response to bortezomib-based therapy
Pol Arch Intern Med, 127; 392‑400 (2017)

 

4. R. M. Thompson, D. Dytfeld, L. Reyes, R. M. Robinson, B. Smith, Y. Manevich,
A. Jakubowiak, M. Komarnicki, A. Przybylowicz-Chalecka, T. Szczepaniak, A. K. Mitra,
B. G. Van Ness, M. Luczak, N. G. Dolloff
Glutaminase inhibitor CB-839 synergizes with carfilzomib in resistant multiple myeloma cells
Oncotarget, 8; 35863-35876 (2017)

 

5. D. Dytfeld, M. Luczak, T. Wrobel, L. Usnarska-Zubkiewicz, K. Brzezniakiewicz,
K. Jamroziak, K. Giannopoulos, A. Przybylowicz-Chalecka, B. Ratajczak, J. Czerwinska-Rybak, A. Nowicki, M. Joks, E. Czechowska, M. Zawartko, T. Szczepaniak, N. Grzasko,
M. Morawska, M.Bochenek, T. Kubicki, M. Morawska, K. Tusznio, A. Jakubowiak,
M. Komarnicki
Comparative proteomic profiling of refractory/relapsed multiple myeloma reveals biomarkers involved in resistance to bortezomib-based therapy.
Oncotarget, Vol. 7, No. 35: 56726-56736 (2016)

 

6. M. Bodnar, M. Luczak, K. Bednarek, L. Szylberg, A. Marszalek, R. Grenman, K. Szyfter, M. Jarmuz-Szymczak, M. Giefing
Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a potential biomarker of metastasis in laryngeal squamous cell carcinoma
Amino Acids 48(6):1469-1476 (2016)

 

7. SJ. Gadher, Ł Marczak, M. Łuczak, M Stobiecki, P Widlak, H Kovarova
Proteomic landscape in Central and Eastern Europe: 9th Central and Eastern European Proteomic Conference, Poznań, Poland.
Expert Review of Proteomics. 13(1): 5-7 (2016)

 

8. J. Cieslak, P. Pawlak, L. Wodas, A. Borowska, A. Stachowiak, K. Puppel, B. Kuczynska,
M. Luczak, L. Marczak, M. Mackowski
Characterization of equine CSN1S2 variants considering genetics, transcriptomics and proteomics
Journal of Dairy Science. 99(2):1277-85 (2016)

 

9. M. Luczak M, J. Suszynska-Zajczyk, L. Marczak, D. Formanowicz, E. Pawliczak,
M. Wanic-Kossowska, M. Stobiecki
iTRAQ-based proteomic analysis of plasma reveals abnormalities in lipid metabolism proteins in chronic kidney disease-related atherosclerosis
Scientific Reports, 6: 32511 (2016)

 

10. M. Luczak M, J. Suszynska-Zajczyk, L. Marczak, D. Formanowicz, E. Pawliczak, M. Wanic-Kossowska, M. Stobiecki
Label-Free quantitative proteomics reveals differences in molecular mechanism of atherosclerosis related and non-related to chronic kidney disease.
International Journal of Molecular Sciences, 17(5). pii: E631 (2016)

 

11. M. Luczak, W. Krzeszowiec-Jeleń, D. Konopka-Postupolska, P.Wojtaszek
Collagenase as a new, useful tool for analysis of plant cellular peripheries.
Phytochemistry.112; 195-209 (2015)

 

12. M. Luczak, Ł. Marczak, D. Formanowicz, E. Pawliczak, M. Wanic-Kossowska,
M. Figlerowicz, M. Stobiecki
Deeper insight into chronic kidney disease-related atherosclerosis: comparative proteomic studies of blood plasma using 2DE and mass spectrometry
Journal of Translational Medicine. 13:20. (2015)