Department of Plant Molecular Physiology
dr Wanda Biała-Leonhard
Aleksandra Pawela, MSc
Tomasz Jamruszka, MSc;
Konrad Pakuła, MSc
ABC transporters, legume plants, Medicago truncatula, secondary metabolites, signaling, symbiosis, mycorrhiza, nodulation, biotechnology, genetic engineering
The Department of Plant Molecular Physiology represents an interdisciplinary unit with its major scientific interests devoted to gene organization and function, characterization of active transport systems in higher plants, analysis of proteins and nucleic acids, bioinformatics and phytochemistry. Our major interest is in the identification and elucidation of the function of plant ABC-type proteins. This numerous transporters play an important role in modulation of plant environment interactions being a part of plant basal immune system implicated in the secretion of defensive/signaling secondary metabolites/molecules. We have performed a systematic search for genes coding putative ABC transporters in Medicago truncatula. At current state of art more than 30 genes coding the so called full size MtABCG transporters have been identified. We are conducting the functional analyses of selected Medicago ABCGs with molecular biology tools. Moreover we are developing transformation facilities, in vitro protocols, and applying chromatography (HPLC, LC-MS) for metabolomic studies in order to understand the role of transport systems like ABC proteins in the distribution of plant natural products.
Current research activity:
Most important research achievements:
Evidence that the full-size ABCG protein is involved in the translocation of early intermediates from the phenylpropanoid biosynthetic pathway.
Presenting ABCGs as modulators of isoflavonoid levels in legumes during biotic stress.
Actual research projects:
Wanda Biała, Joanna Banasiak, Karolina Jarzyniak, Aleksandra Pawela, Michał Jasiński Medicago truncatula ABCG10 is a transporter of 4-coumarate and liquiritigenin in the medicarpin biosynthetic pathway JEXBOT-2016-191973 ID: erx059
M. Suzuki, R. Nakabayashi, Y. Ogata, N. Sakurai, T. Tokimatsu, S. Goto, M. Suzuki, M. Jasinski, E. Martinoia, S. Otagaki, S. Matsumoto, K. Saito, K. Shiratake Multi omics in grape berry skin revealed specific induction of stilbene synthetic pathway by UV-C irradiation. Plant Physiol. 114.254375, 2015.
K.M. Jarzyniak, M. JasińskiMembrane transporters and drought resistance - a complex issueFrontiers in Plant Science 5:687, 1-15, doi: 10.3389/fpls.2014.00687 (2014).
J. Banasiak, M. JasińskiDefence, symbiosis and ABCG transporters. W: Plant ABC Transporters; Signaling and Communication in Plants Springer International Publishing Switzerland 22, 163-184 (2014).
J. Banasiak, W. Biała, A. Staszków, B. Swarcewicz, E. Kępczyńska, M. Figlerowicz, M. Jasiński A Medicago truncatula ABC transporter belonging to subfamily G modulates the level of isoflavonoids. Journal of Experimental Botany 64, 1005-15 (2013).
Jasinski M, Banasiak J, Radom M, Kalitkiewicz A, Figlerowicz M. Full-size ABC transporters from the ABCG subfamily in medicago truncatula. Mol Plant Microbe Interact. 2009 Aug;22(8):921-31