Cell and Tissue Culture Laboratory


 

Head:
Dr. Paweł M. Stróżycki

 

 


                       

Staff:

Dr. Mariola Piślewska-Bednarek

Nelli Malinowska Eng.

Hanna Glapiak

Magdalena Puszczyk

 

 

 

 

Research proposal:

The Cell and Tissue Culture Laboratory (CTCL) is a platform that provides specialized equipment and conditions (sterility, controlled temperature, humidity etc.) for conducting (highly complex) experiments in the scope of a broadly defined area of molecular biology with the use of cultures of cell lines and tissues. The Laboratory’s infrastructure and equipment ensures safe work with animal as well as plant origin material.   A self-contained area for experiments involving virus type material is also available. The character of the Laboratory allows performing full-cycle experiments, from the propagation of the biological material through putting it to use in experiments, up to obtaining material for further analyses outside the CTCL block. Once a special permit is obtained, work with transgenic material is also allowed. The microscopic as well as photographic accessories which are also part of the Laboratory’s equipment, allow maintenance of a full scientific documentation.

The CCTL provides services (for a fee) which include realization of external projects and services within the scope of experimental biology.

 

The Laboratory’s units include: Animal, Virus, Plant and Microscopic imaging workrooms

 

Keywords:  cell cultures, tissue cultures, transfection, micro-bombardment, ...

 

 

Equipment/Accessories:

The Cell and Tissue Culture Laboratory block meets the requirements of the BSL2 biosafety regulations.

All the Laboratory’s units are equipped with the state of art equipment typical for molecular biology laboratories (freezers, liquid nitrogen storage tanks, centrifuges, water baths, automatic pipetors...). Standard workstations are laminar flow cabinets, of which some include BioHazard protection (Alpina, Steril) and waste collecting (Vacusafe-Integra) security systems.  Units are equipped with air shaker-incubators (New Brunshwick, Innowa ) and incubators for growing biological material in a carbon dioxide atmosphere (Memmert).

Additionally, the plant unit has dedicated controlled environment tissue culture rooms and plant growth chambers (Percival E-41E with LED illumination). The Biolistic Particle Delivery System (BioRad PDS-1000/He) is also installed there. This culture and growth section is additionally equipped with a computer controlled phytotron cluster.

The Laboratory’s infrastructure is supplemented with separate, for sterile complex and phytotron, preparation cubicles.

All laboratory premises and equipment are subject to restrictive sterility rules (UV sterilization systems, ozonation).

The Cell and Tissue Culture Laboratory is also a supported by a Microscopic Imaging unit which among other things has at its disposal an inverted fluorescence microscope Leica DM IL LED with a CoolLED illumination system and a stereoscopic microscope Leica M205 FA. Both microscopes are equipped with documentation systems and specialized software. A macrophotography workstation is also in place. Further to this, next to laminar flow cabinets, hand-held microscopes are also accessible.

 

 

 

Current research activities:

Twelve projects in which animal cells are employed are currently in progress, and there are nine other projects based on the use of plant cell cultures. Below is a list of the main projects indicating the major research areas for which the Laboratory’s resources may be used:

 

Structure defined oligonucleotides for revealing fundamental insights into structure and function of RNAs

 ICHB PAN; NCN - MAESTRO

 

In vivo and in vitro structure of influenza virus RNA. The structural determinants behind the inhibition of influenza virus proliferation with modified antisense oligonucleotides, siRNAs and small molecule ligands.
ICHB PAN; NCN – OPUS

Conserved RNA structural motifs in influenza virus: toward revealing viral RNA functions

ICHB PAN; NCN - HARMONIA

 

The role of the 5’ non-coding regions of p53 mRNA transcripts, which are synthesized from various transcription promoters and which use alternative translation initiation sites in generating p53 protein isoforms

ICHB PAN; NCN - OPUS

 

Structural studies of proteins crucial for tick-mammal-pathogen interactions.
ICHB PAN; NCN – OPUS

 

Establishment of a novel scientific workshop enabling complex analysis of RNA degradome - elaboration of innovative methods for the identification of RNA degradants and the assessment of their functional potential

ICHB PAN; NCN - SONATA

 

Evolutionary modeling physicochemical conditions for overexpression of viral replicons in plant liquid cultures

ICHB PAN; NCN – SONATA

 

Low molecular weight epigenetic modulators for activation of pluripotency of cells for regenerative medicine purposes

EPICELL; ICHB PAN; NCBR – STRATEGMED

 

Thermodynamic insights into design of functionally optimized modified siRNAs and their experimental verification

ICHB PAN; NCN - SONATA BIS

 

Defining new neurodegeneration mechanisms using the first SCA3/MJD knock-in mouse model.

ICHB PAN            NCN - SONATA BIS

 

Impact of noncoding RNAs on renal cell development and carcinogenesis

ICHB PAN; NCN - PRELUDIUM

 

The Role of JMJD3 in cancer

ICHB PAN

 

Producing a fragment of Dicer exhibiting potential DNase activity in bacterial expression systems

ICHB PAN

 

Identification of molecular mechanisms involved in soybean's cold stress response in

temperate climate conditions

ICHB PAN; NCN – OPUS

 

Molecular mechanisms underlying loss of a defensive metabolic pathway in a phylogenetic clade of plant species closely related to Capsella rubella.

ICHB PAN; NCN – OPUS

 

Plant secondary metabolites controlling microbial colonizers.

ICHB PAN; NCN - SONATA BIS

 

The role of membrane transporters in the cytokinin signaling upon Legume- Rhizobia Symbiosis

ICHB PAN; NCN – OPUS

 

ABA transport in legumes - between drought stress responses and nitrogen fixation efficiency

ICHB PAN; NCN - HARMONIA

 

Identification of circular RNAs and protein factors involved in their biogenesis in a model plant, Arabidopsis thaliana

ICHB PAN; NCN – SONATA

 

Active transport systems as an element that controls the molecular cross-talk between symbionts during arbuscular mycorrhiza formation

ICHB PAN; NCN - SONATA

 

Elucidation of the mechanisms that induce copy number polymorphism of large genome fragments and of the relations between this phenomenon and metabolic gene cluster formation and evolution in plants.

ICHB PAN; NCN, OPUS

 

Analysis of two metabolic pathways: 1 - monolignol synthesis, 2 – sucrose photosynthetic synthesis

ICHB PAN