Department of Molecular Neurooncolgy


 

Kierownik:

Katarzyna Rolle, PhD;

Associate Prof. IBCH PAS

 


Staff:

Dariusz Wawrzyniak, MSc

 Latowska Juia, MSc

 


PhD Students:

Paweł Głodowicz, MSc;

Małgorzata Grabowska, MSc;

Konrad Kuczyński, MSc;

Żaneta Zarębska, MSc;

 


Affiliated professors:

Prof. Jan Barciszewski


Rys.1

 

Rys.2

 

Rys.3

 

Rys.4

Keywords:

brain tumors, glioblastoma multiforme, cancer development and progression, cancer stem cells, regulatory RNAs, circular RNAs, miRNAs, extracellular matrix, epithelial-mesenchymal transition

 

Research area:

The main goal of the Department of Molecular Neurooncology is the complex research of the basics of development and progression of brain tumors, especially glioblastoma multiforme (GBM). Our interests include mainly:

- the identification and characteristics of the regulatory RNAs crucial for the cancer development, such as  miRNA, lncRNA and circRNA, especially their importance for cancer stem cells and processes like: unlimited cell divisions, ability to form secondary tumors,
or eventually resistance to chemotherapy,

- the establishing the role of extracellular matrix (ECM) components in metastases, resistance to treatment and processes associated with epithelial-mesenchymal transition,

-the identification of RNA molecules with potential diagnostic, prognostic and therapeutic significance.

In our experimental approach we use a combination of molecular biology tools, in vivo imaging techniques with functional studies using clinical tissue samples, human cell lines (including primary tumor cells isolated from patients) and syngeneic mouse tumor models.


Ongoing research projects:

1. Functional crosstalk between circRNAs and extracellular matrix (ECM) - the importance for glioblastoma stem cells (GSC) and epithelial-mesenchymal transition (EMT) in brain tumors
NCN – OPUS 13

2.The characteristics of the regulatory RNAs landscape in glioblastoma multiforme (GBM). Circular RNAs and micro RNA-like molecules as a new players in gilomagenesis and GBM progression and their importance for glioblastoma stem cells
NCN – SONATA BIS 7

3. Catalytic RNAs as a tool for human mitochondrial biotechnology.
NCN – PRELUDIUM 11

4. New, multifunctional nanoparticles for combined photothermal and gene silencing cancer therapy
NCN – OPUS 11

 

Selected publications from the last three years:

1. Szymańska-Michalak A, Wawrzyniak D, Framski G, Stawiński J, Barciszewski J, Kraszewski A.
New antiglioma zwitterionic pronucleotides with a FdUMP framework.
Eur. J. Med. Chem. 144:682-691 (2018)

2. Belter A, Rolle K, Piwecka M, Fedoruk-Wyszomirska A, Naskręt-Barciszewska MZ, Barciszewski J
Inhibition of miR-21 in glioma cells using catalytic nucleic acids.
Sci. Rep. 15; 6:24516 (2016)

3. Rolle K, Piwecka M, Belter A, Wawrzyniak D, Jeleniewicz J, Barciszewska MZ, Barciszewski J
The sequence and structure determine the function of mature human miRNAs
PLOS One 11(3), e0151246 (2016)

4. Sultan LD, Mileshina D, Grewe F, Rolle K, Abudraham S, Głodowicz P, Niazi AK, Keren I, Shevtsov S, Klipcan L, Barciszewski J, Mower JP, Dietrich A, Ostersetzer-Biran O
The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria
Plant Cell 28:2805-2829 (2016)

5. Framski G, Wawrzyniak D, Jahnz-Wechmann Z, Szymańska-Michalak A, Kraszewski A, Barciszewski J, Boryski J, Stawiński J.
Searching for anti-glioma activity. Ribonucleoside analogues with modifications in nucleobase and sugar moieties.
Acta Biochim. Pol. 63:765-77 (2016)

6. Szymańska-Michalak A, Wawrzyniak D, Framski G, Kujda M, Zgoła P, Stawiński J, Barciszewski J, Boryski J, Kraszewski A.
New 3’-O-aromatic acyl-5-fluoro-2’-deoxyuridine derivatives as potential anticancer agents.
Eur. J. Med. Chem. 115:41-52 (2016)

7. Piwecka M, Rolle K, Belter A, Barciszewska AM, Żywicki M, Michalak M, Nowak S, Naskręt-Barciszewska MZ, Barciszewski J
Comprehensive analysis of microRNA expression profile in malignant glioma tissues
Mol Oncol. 9:1324-40 (2015)

8. Rolle K
miRNA multiplayers in glioma. From bench to bedside
Acta Biochim Pol. 62: 353-365 (2015)