|
The Laboratory's current aim is to understand the structure, function and interactions of the functionally important domains of RNA on atomic and molecular level.
The idea is to combine, within the Laboratory, chemistry, molecular biology, structural and calculation lines of research in the RNA field. The chemical / in vitro synthesis, [13C, 15N, fluorine]- and fluorophore- labelling are dedicated to the RNA structural studies using molecular biology approaches of the structural probing, heteronuclear NMR spectroscopy, molecular dynamics and other calculation metods.
Recently, two groups emerged within the Laboratory: Biomolecular NMR Group (Zofia Gdaniec) and Biomolecular Modelling Group (Tadeusz Kuliński). Also, after returning from his postdoc, Mikołaj Olejniczak started a new research project on small bacterial RNAs. Our particular areas of interest include: (i) structure of retroviral RNAs (HIV-1, HIV-2), their interactions with metal ions and recombinant proteins (Tat-2, cyclin T1) and the accessibility in view of RNAi applications, (ii) structure and function of non-coding RNAs in the pathogenic bacteria, (iii) NMR structure of non-helical RNA regions, 2'-O-MeRNA / LNA chimera duplexes, RNA triplet repeats, Z-RNA and (iv) RNA dynamics and developing new MD approaches in a view of RNA structure and specificity of protein recognition and methods directed toward 3D RNA structure prediction.
|
M. Popenda, M. Blazewicz, M. Szachniuk and R.W. Adamiak, RNA FRABASE version 1.0: an engine with a database to search for the three-dimensional fragments within RNA structures.
Nucleic Acids Research, 36, D386-D391 (2008). (published online on October 5, 2007) [pdf] [pdf]
|
